Joshua Rykiel ’25, Luke Neri ’25, and Ryan Cielo ’25
Stuck Between a Cow and a Plant Place; Analysis of EHEC Attachment Mechanisms on Bovine Cells and Plants
Joshua Rykiel ’25, Biology major
Luke Neri ’25, Biology major
Ryan Cielo ’25, Biology major
Faculty mentor: Dr. Matthew R. Moreau
Enterohemorrhagic Escherichia coli (EHEC) is a dangerous E. coli pathovar due to its wide host range and Shiga toxin production, which damages blood vessels. Super shedder (SS) cattle shed EHEC at significantly higher rates, contributing to environmental contamination. SS isolates exhibit strong biofilm formation and adhesion to bovine recto-anal junction epithelial cells. To investigate genetic factors influencing attachment, we analyzed SS and non-SS isolates on leafy greens. Our findings suggest attachment and biofilm formation are strain, host, and temperature dependent, highlighting yfaL as a key factor conserving phenotype across both SS and non-SS isolates. This research aids in identifying potential therapeutic targets.
Poster Presentation: Wednesday, April 23, 11 a.m. – 12:30 p.m.
